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Autologous blood clot characteristics and secretome comparison between healthy control ( n = 12) and metabolic syndrome/diabetic (MetS/DM) ( n = 10) participants. (A) Representative image illustrating the blood clot in culture (that was spiked in with the respective treatments prior to clot induction) and its secretome. (B) The ratio of the initial clot size (surface area) and total protein (μg/mL) released into the clot secretome within the respective treatment groups. (C–D) Quantification <t>of</t> <t>PDGF‐BB</t> (pg/mL) (C), P‐selectin (pg/mL) (D) and CCL‐5 (pg/mL) levels within the clot secretome within the respective treatment groups. Statistical analysis: Two‐way ANOVA with Sidaks multiple comparisons test. * p < 0.05, ** p < 0.01.
Platelet Derived Growth Factor Bb Pdgf Bb, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human platelet derived growth factor  (R&D Systems)
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Autologous blood clot characteristics and secretome comparison between healthy control ( n = 12) and metabolic syndrome/diabetic (MetS/DM) ( n = 10) participants. (A) Representative image illustrating the blood clot in culture (that was spiked in with the respective treatments prior to clot induction) and its secretome. (B) The ratio of the initial clot size (surface area) and total protein (μg/mL) released into the clot secretome within the respective treatment groups. (C–D) Quantification <t>of</t> <t>PDGF‐BB</t> (pg/mL) (C), P‐selectin (pg/mL) (D) and CCL‐5 (pg/mL) levels within the clot secretome within the respective treatment groups. Statistical analysis: Two‐way ANOVA with Sidaks multiple comparisons test. * p < 0.05, ** p < 0.01.
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human platelet growth factor  (Thermo Fisher)
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Autologous blood clot characteristics and secretome comparison between healthy control ( n = 12) and metabolic syndrome/diabetic (MetS/DM) ( n = 10) participants. (A) Representative image illustrating the blood clot in culture (that was spiked in with the respective treatments prior to clot induction) and its secretome. (B) The ratio of the initial clot size (surface area) and total protein (μg/mL) released into the clot secretome within the respective treatment groups. (C–D) Quantification <t>of</t> <t>PDGF‐BB</t> (pg/mL) (C), P‐selectin (pg/mL) (D) and CCL‐5 (pg/mL) levels within the clot secretome within the respective treatment groups. Statistical analysis: Two‐way ANOVA with Sidaks multiple comparisons test. * p < 0.05, ** p < 0.01.
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human platelet derived growth factor hpdgf aa  (Thermo Fisher)
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Autologous blood clot characteristics and secretome comparison between healthy control ( n = 12) and metabolic syndrome/diabetic (MetS/DM) ( n = 10) participants. (A) Representative image illustrating the blood clot in culture (that was spiked in with the respective treatments prior to clot induction) and its secretome. (B) The ratio of the initial clot size (surface area) and total protein (μg/mL) released into the clot secretome within the respective treatment groups. (C–D) Quantification <t>of</t> <t>PDGF‐BB</t> (pg/mL) (C), P‐selectin (pg/mL) (D) and CCL‐5 (pg/mL) levels within the clot secretome within the respective treatment groups. Statistical analysis: Two‐way ANOVA with Sidaks multiple comparisons test. * p < 0.05, ** p < 0.01.
Human Platelet Derived Growth Factor Hpdgf Aa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human platelet derived growth factor bb  (R&D Systems)
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R&D Systems recombinant human platelet derived growth factor bb
Autologous blood clot characteristics and secretome comparison between healthy control ( n = 12) and metabolic syndrome/diabetic (MetS/DM) ( n = 10) participants. (A) Representative image illustrating the blood clot in culture (that was spiked in with the respective treatments prior to clot induction) and its secretome. (B) The ratio of the initial clot size (surface area) and total protein (μg/mL) released into the clot secretome within the respective treatment groups. (C–D) Quantification <t>of</t> <t>PDGF‐BB</t> (pg/mL) (C), P‐selectin (pg/mL) (D) and CCL‐5 (pg/mL) levels within the clot secretome within the respective treatment groups. Statistical analysis: Two‐way ANOVA with Sidaks multiple comparisons test. * p < 0.05, ** p < 0.01.
Recombinant Human Platelet Derived Growth Factor Bb, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Autologous blood clot characteristics and secretome comparison between healthy control ( n = 12) and metabolic syndrome/diabetic (MetS/DM) ( n = 10) participants. (A) Representative image illustrating the blood clot in culture (that was spiked in with the respective treatments prior to clot induction) and its secretome. (B) The ratio of the initial clot size (surface area) and total protein (μg/mL) released into the clot secretome within the respective treatment groups. (C–D) Quantification of PDGF‐BB (pg/mL) (C), P‐selectin (pg/mL) (D) and CCL‐5 (pg/mL) levels within the clot secretome within the respective treatment groups. Statistical analysis: Two‐way ANOVA with Sidaks multiple comparisons test. * p < 0.05, ** p < 0.01.

Journal: Wound Repair and Regeneration

Article Title: Autologous Blood Clot Therapy for Wounds: Investigating the Chemotactic Effect on PBMCs and Fibroblasts in Diabetes

doi: 10.1111/wrr.70153

Figure Lengend Snippet: Autologous blood clot characteristics and secretome comparison between healthy control ( n = 12) and metabolic syndrome/diabetic (MetS/DM) ( n = 10) participants. (A) Representative image illustrating the blood clot in culture (that was spiked in with the respective treatments prior to clot induction) and its secretome. (B) The ratio of the initial clot size (surface area) and total protein (μg/mL) released into the clot secretome within the respective treatment groups. (C–D) Quantification of PDGF‐BB (pg/mL) (C), P‐selectin (pg/mL) (D) and CCL‐5 (pg/mL) levels within the clot secretome within the respective treatment groups. Statistical analysis: Two‐way ANOVA with Sidaks multiple comparisons test. * p < 0.05, ** p < 0.01.

Article Snippet: The concentrations of platelet‐derived growth factor‐BB (PDGF‐BB) (E‐EL‐H1577, Elabscience, USA), P‐selectin (SEA569Hu; Cloud‐Clone Corp, USA) and CCL‐5 (Proteintech, USA) were determined using sandwich ELISA assays and the absorbance read at 450 nm using a microplate reader (Multiskan GO 1.00.40; Thermo‐Scientific Group, USA) according to the manufacturer's instructions.

Techniques: Comparison, Control